ISOLATION AND RADIOIMMUNOASSAY OF THYROXINE-BINDING GLOBULIN (TGB)

Thyroxine-Binding Globulin Concentrations

Plasma thyroid hormone concentrations may be abnormal in non-thyroid disease and pose diagnostic problems. For example, concentrations of triiodothyronine (T3) in the blood decline in acute illness with a reciprocal increase in reverse triiodothyronine (reverse T3), but thyroxine (T4) concentrations are usually normal or only slightly decreased (1, 2). However, McLarty et al. (3) reported that ...

Familial thyroxine-binding globulin deficiency.

Genetically determined thyroxine-binding globulin deficiency is described in two families in the United Kingdom. All subjects in both pedigrees were euthyroid. Transmission was by sex-linkage; males showed low serum protein bound iodine and high thyroxine (T4) resin uptake due to complete absence of serum thryroxine-binding globulin; females were less severely affected. The distinctive biochemi...

Determination of thyroxine-binding globulin in human serum by single radial immunodiffusion and radioimmunoassay.

Two immunochemical methods for determination of thyroxine-binding globulin in human serum were developed, in which the purified globulin and monospecific antiserum to it are used. One method, based on radial immunodiffusion, has good precision and values for analytical recovery. Reference values obtained for men were 9.8-17.8 mg/liter and for women 11.3-20.5 mg/liter. The sex-related difference...

Interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin.

Although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. We investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. Carrier-free 125I-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free frac...

Thyroxine-binding globulin, hyperthyroxinemia and hepatocellular carcinoma.